This MS/MS library has been set up by injection of reference compounds over a Phenomenex Synergi Polar-RP column (50 × 2.00 mm, 4 µm) with looped MS/MS experiments using collision energies of 20, 35 and 50 eV respectively, as well as a collision energy spread of 35 ± 15 eV. Thus, four spectra of each compound’s quasimolecular ion were acquired.
In general, MS/MS spectra are very reproducible, if scan parameters are similar (step-size: 0.1 amu, resolution: unit). Acceptable search results can be achieved with the mass spec software Analyst 1.4 with LibrarySearchEnginePatch1.4 or higher versions of Analyst
The library contains the following information on the compounds: